Project 134: Construction of Primary Cell Lineage and Human Transplanted Tumor Model of Tumor Tissue
Contact Information:
Prof. Liming Lu
Email: lulunew2003@163.com
Project Description and Objectives:
Collect fresh lung cancer specimens, and carry out the following:
1. Primary cell culture and identify cell lines, including STR detection, phase contrast microscopy of tumor cells, identification of HE and immunohistochemical staining, plotting tumor cell growth curve, cell cycle analysis, tumorigenic observation, karyotype analysis, etc.
2. Construction of human xenograft models: Humanized tumor models will be constructed and transplanted into immunodeficient mice by patient-derived ones. These tumor models will be used to deeply study the mechanism of tumorigenesis and to understand the pharmacology of drugs in tumor tissues of patients as well as pharmacodynamic response, guiding patients to clinical medication.
Eligibility Requirements:
Basic knowledge of medicine or biology.
Main Tasks:
Primary cell culture and identify cell lines.
Construction of human xenograft models.
Website
School: N/A