Project 133: Application of IL-38 in Precise Target Therapy of Colorectal Cancer based on Single Cell Analysis and Clinicopathological Study

Contact Information:

Prof. Yongchun Yu

Email: yyc2166@sjtu.edu.cn

Project Description and Objectives:

Ferroptosis is a type of programmed cell death which is tightly associated with cell metabolism. Overload of lipid ROS would lead to cell ferroptosis. However, the ferroptosis status of cells and whether ferroptosis inducing could be used as a tumor treatment strategy in lung cancer remains to be further investigated. Our laboratory will search for target genes and target proteins related to ferroptosis through high-throughput combined bioinformatics methods and we will explore relevant mechanisms in cell lines and primary cells and finally verify the conclusions in tumor specimens and transgenic animals. We anticipate to find a series of target genes, target proteins and metabolites related to ferroptosis and understand the mechanism of ferroptosis promotion or inhibition in lung cancer. We also anticipate exploring whether ferroptosis might be used as a new type of treatment for lung cancer and find the relevant potential therapeutic targets. In this project, the participant will perform various types of molecular biology experiments, including Western Blotting, immunohistochemistry, immunofluorescence, real-time fluorescent quantitative PCR, molecular cloning, transgenic animal manipulation, and clinical specimen testing. In addition, the participant will understand the classical molecular mechanism of ferroptosis and have research ideas on ferroptosis studies and other basic research related to metabolism or signaling pathways.

Eligibility Requirements:

Basic knowledge of molecular biology.

Preferred: Experience in biology and medical research.

Main Tasks:

Gaining proficiency in Western Blotting, real-time fluorescent quantitative PCR, animal experiments, immunohistochemistry, immunofluorescence and other molecular experiments, and basic research ideas for ferroptosis.

Website:

Lab: http://www.shxkyy.com

School: N/A