Project 129: Airborne Microbial Communities in Shanghai Neighborhoods: Responses to Garden Age and Urban-rural Gradient
Contact Information:
Assoc. Prof. Yang Luan
Email: yluan@sjtu.edu.cn
Project Description and Objectives:
Aristolochic acids (AAs) are a kind of compounds with nitrophenanthrene core structure, derived from the Aristolochiaceae family of plants. AAs-containing herbs have been used medicinally for thousands of years. Until a few decades ago, the health risk of AAs raised great concern because of their nephrotoxicity, genotoxicity, and carcinogenicity. We will evaluate the genotoxicity of representative AAs with (Quantitative) structure-activity relationship ((Q)SAR), micronucleus assay and Comet assay in a human induced Hepatocytes (hiHeps). We also plan to detect mitochondrial dysfunction via JC-1 assay.
Eligibility Requirements:
l Basics in molecular technology, ecology and statistics.
l Good communication skills in English.
Main Tasks:
l Environmental microbial sampling and physical-chemical analyses.
l Total DNA extraction, rRNA gene amplification, qPCR and Illumina Miseq sequencing.
l Bioinformatics and statistical analysis, R, Mothur and JMP
Website:
Lab: https://www.agri.sjtu.edu.cn/Data/View/4605
School: https://www.agri.sjtu.edu.cn