Project 129: Genetic Toxicity Evaluation of Human Induced Hepatocytes (hiHeps) Induced by Aristolochic Acid

Contact Information:

Assoc. Prof. Yang Luan   

Email: yluan@sjtu.edu.cn

Project Description and Objectives:

Aristolochic acids (AAs) are a kind of compounds with nitrophenanthrene core structure, derived from the Aristolochiaceae family of plants. AAs-containing herbs have been used medicinally for thousands of years. Until a few decades ago, the health risk of AAs raised great concern because of their nephrotoxicity, genotoxicity, and carcinogenicity. We will evaluate the genotoxicity of representative AAs with (Quantitative) structure-activity relationship ((Q)SAR), micronucleus assay and Comet assay in a human induced Hepatocytes (hiHeps). We also plan to detect mitochondrial dysfunction via JC-1 assay.

Eligibility Requirements:

Fundamentals of chemistry and biology.

Main Tasks:

Understand the principle of genetic toxicity evaluation.

Website:

Lab: N/A

School: https://www.shsmu.edu.cn/sph-en/