Project 124: Functional Analysis of Proteins Interacting with the Key Factor of Light Signaling Pathway
Contact Information:
Email: ruohe.yin@sjtu.edu.cn
Project Description and Objectives:
Light is a key environmental factor affecting plant growth and development. Photoreceptors can sense light signals and trigger downstream signal transduction pathways, which regulate many physiological processes in plants. The objective of this project is to screen the interacting proteins of the important factor in the light signal transduction pathway, and investigate the function of the interacting proteins. The results will contribute to our further understanding in light regulating plant growth and development.
Eligibility Requirements:
Applicants should have basic knowledge of genetics, molecular biology and basic biochemistry.
Experience in biological research would be preferred.
Able to document experimental results and draw conclusions.
Main Tasks:
To perform protein-protein interaction assays with Y2H, BiFC etc.
To grow and verify the transgenic plant lines and mutants by genomic PCR, RT-PCR and western-blot.
To observe and document the phenotypes of the transgenic lines and mutants.
To analyze protein subcellular localization by confocal microscope.
Website:
Lab: https://rhyin.sjtu.edu.cn/