Project 113: Cloning and Functional Characterization of Rice Male Sterile Genes
Contact Information:
Prof. Wanqi Liang
Email: wqliang@sjtu.edu.cn
Project Description and Objectives:
The life cycle of flowering plants alternates between diploid sporophyte and haploid gametophyte generations. Male gametophytes develop in the anther compartment of the stamen within the flower which requires cooperative functional interactions between gametophytic and sporophytic tissues. The male reproductive development is highly complicated, involving numerous biological events, including cell division, differentiation and degeneration of somatic tissues consisting of four concentric cell layers surrounding and supporting reproductive cells as they form mature pollen grains through meiosis and mitosis. To understand the mechanism of plant male reproduction, we are combining systematic biology (genomics, transcriptomics, proteomics, metabonomics) with other approaches such as genetics, cell biology, biochemistry, and structural biology to elucidate the molecular mechanisms underlying each biological event of male reproduction.
Eligibility Requirements:
Applicants should have basic knowledge of biology.
Experience in biological research would be an advantage.
Main Tasks:
Learn the methods of anther and pollen observation, perform DAPI staining, semi-thin section and SEM observation.
Learn the principle of gene cloning by map-based cloning and Mut-map analysis, perform plant DNA extraction and PCR detection.
Learn the methods of bioinformatic analysis of gene functions, perform phylogenetic analysis and RNA-seq data analysis.
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