Project 97: Liquid Sampling Protein Western Blotting to Replace Traditional Labor Intensive Western Blotting

Contact Information

Prof. Xianting Ding

Project Description and Objectives

Single Cell Western Blotting (scWB) is powerful method for high resolution single cell protein analysis, as the technique reports both target molecular mass (via protein electrophoresis) and probe binding (via subsequent antibody probing), not simply probe binding. In recent years, various techniques have been developed based on scWB, including but not limited to: 3D projection electrophoresis for single cell immunoblotting, multi-detection of protein isoforms and nucleic acid by scWB, DropBlot: single-cell western blotting of chemically fixed cancer cells… However, the core principle of the technique involves single-cell loading, followed by in-well cell lysis and electrophoresis, which makes it poorly suited for liquid protein samples analysis. In this project, we aim to develop a novel Western blotting-based technology, called 'Liquid Protein Sample Western Blotting,' for analyzing liquid samples. This approach can potentially serve as a faster, simpler, and more sensitive alternative to traditional Western blotting.

Eligibility Requirements

The students should have an interest in microfluidics and single cell proteomics analysis and should have a background in biotechnology, microfluidics, and analytical chemistry.

Main Tasks

The students are required to cooperate with other graduate students under the supervision of their mentor(s) to work on the above projects. The students need to attend regular lab meetings and make weekly reports to their mentor(s). By the end of the internship a project report should be submitted.

Website

Lab: www.dinglab.com.cn

School: http://ipmsjtu.sjtu.edu.cn/