Project 132: Mechanisms of Long Non-coding RNA FENDRR Affecting Metastasis of Colon Cancer by Regulating PBXIP1 Expression

Contact Information:

Prof. Kun Tao      

Email: taokun20119@163.com

Project Description and Objectives:

Mechanisms of long non-coding RNA FENDRR affect the metastasis of colon cancer by regulating PBXIP1 expression. In this study, we will study the changes of FENDRR and PBXIP1 gene and protein expression in colon cancer cell lines and fresh tissues and the changes of invasiveness of colon cancer cell lines before and after gene knockout, so as to reveal the relationship between FENDRR and PBXIP1 and the mechanism of colon cancer metastasis.

Eligibility Requirements:

Students should master some experimental techniques such as cell culture, tissue preparation, immunohistochemistry, FISH, RT-PCR, Western blot and so on. 

Main Tasks:

Starting with cell culture, protein extraction, gene isolation, purification and identification, protein localization and so on, we can help students master the commonly used experimental methods and skills in pathological research. Based on this, students will have an overall grasp of the application and significance of molecular biology methods. What’s more, we will provide a variety of experimental materials for students to design and prepare experiments on their own, so as to cultivate independent scientific research ability.

Website:

Lab: http://blk.shtrhospital.com  

School: http://www.shtrhospital.com