Project 123: Genetic Toxicity Evaluation of Human Induced Hepatocytes (hiHeps) Induced by Aristolochic Acid

Contact Information:

Asso. Prof. Yang Luan

Project Description and Objectives:

Be familiar with the lab, attend and pass the training of new staff, read and memorize the rules and regulations.

Read literature on aristolochic acid and human induced hepatocytes and be familiar with cell culture and passage methods.

Study the genetic toxicity evaluation method and routine combination experiment. It includes the principle, operation, observation and judgment of each experiment.

The cells were treated with aristolochic acid. According to the guiding principles and literatures, the appropriate toxic dose was found through preliminary experiments for the next formal experiment, so as to avoid false positive results caused by excessive toxicity.

According to the pre-experimental dose, a formal experiment was conducted. Get the results of all experiments, analyze the results through statistics and biology, write conclusions and write experimental reports and summaries.

Eligibility Requirements:

Fundamentals of chemistry and biology.

Main Tasks:

Understand the principle of genetic toxicity evaluation.

Website:

Lab: N/A

School: https://www.shsmu.edu.cn/sph-en/