Project 134: Construction of Primary Cell Lineage and Human Transplanted Tumor Model of Tumor Tissue

Contact Information:

Prof. Liming Lu

Email: lulunew2003@163.com

Project Description and Objectives:

Collect fresh lung cancer specimens, and carry out the following:

1. Primary cell culture and identify cell lines, including STR detection, phase contrast microscopy of tumor cells, identification of HE and immunohistochemical staining, plotting tumor cell growth curve, cell cycle analysis, and tumorigenic observation, karyotype analysis, etc.

2. Construction of human xenograft model: Humanized tumor models were constructed and transplanted into immunodeficient mice by patient-derived ones. These tumor models were used to deeply study the mechanism of tumorigenesis and to understand the pharmacology of drugs in tumor tissues of patients as well as pharmacodynamic response, guiding patients to clinical medication.

Eligibility Requirements:

Basic knowledge of medicine or biology.

Main Tasks:

Primary cell culture and identify cell lines.

Construction of human xenograft model.

Website

Lab: http://www.shxkyy.com

School: N/A